验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, Jurkat cells |
| Positive IHC detected in | human endometrial cancer tissue, human cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14946-1-AP targets RPS27A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPS27A fusion protein Ag6750 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S27a |
| 别名 | UBCEP1, UBA80, Small ribosomal subunit protein eS31, ribosomal protein S27a, HUBCEP80 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 9 kDa, 18 kDa |
| GenBank蛋白编号 | BC001392 |
| 基因名称 | RPS27A |
| Gene ID (NCBI) | 6233 |
| RRID | AB_2878093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62979 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS27A antibody 14946-1-AP | Download protocol |
| IHC protocol for RPS27A antibody 14946-1-AP | Download protocol |
| IF protocol for RPS27A antibody 14946-1-AP | Download protocol |
| FC protocol for RPS27A antibody 14946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Chem Integration of RNA-seq and ATAC-seq analyzes the effect of low dose neutron-γ radiation on gene expression of lymphocytes from oilfield logging workers | ||
PLoS One Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters. | ||
Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation | ||
J Pineal Res Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin | ||
Nat Commun Nuclear and cytoplasmic specific RNA binding proteome enrichment and its changes upon ferroptosis induction |
