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CoraLite® Plus 488-conjugated RPS27A Polyclonal antibody

RPS27A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14946

Print datasheet

Synonyms

CEP80, HUBCEP80, ribosomal protein S27a, RPS27A, UBA80, UBCEP1, UBCEP80, ubiquitin

验证数据展示

  • IF Staining of HeLa using CL488-14946

    IF Staining of HeLa using CL488-14946

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 RPS27A antibody (CL488-14946) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14946-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14946 targets RPS27A in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen RPS27A fusion protein Ag6750 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S27a
Synonyms CEP80, HUBCEP80, ribosomal protein S27a, RPS27A, UBA80, UBCEP1, UBCEP80, ubiquitin
Calculated Molecular Weight18 kDa
Observed Molecular Weight 9 kDa, 18 kDa
GenBank Accession NumberBC001392
Gene Symbol RPS27A
Gene ID (NCBI) 6233
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
UNIPROT IDP62979
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS27A, also named as UBA80 and UBCEP1, can be cleaved into two chains Ubiquitin and 40S ribosomal protein S27a. Ubiquitin is a highly conserved 76-amino acid protein that plays a key role in protein degradation. Ubiquitin is synthesized as precursor proteins that consist either of polyubiquitin chains that are cleaved into moieties of the UBB or UBC types, or single ubiquitin moieties fused 5-prime to unrelated carboxyl extension proteins (UBA type) such as UBA52 and UBA80 (RPS27A) [PMID:10772958]

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RPS27A antibody CL488-14946Download protocol
Standard Protocols
Click here to view our Standard Protocols