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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80992-1-RR (same clone as 80992-1-PBS)

    WB analysis using 80992-1-RR (same clone as 80992-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80992-1-RR (ubiquitin antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 80992-1-RR (same clone as 80992-1-PBS)

    IHC staining of human pancreas cancer using 80992-1-RR (same clone as 80992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBB antibody included
  • IHC staining of human lung cancer using 80992-1-RR (same clone as 80992-1-PBS)

    IHC staining of human lung cancer using 80992-1-RR (same clone as 80992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBB antibody included
  • IF Staining of HeLa using 80992-1-RR (same clone as 80992-1-PBS)

    IF Staining of HeLa using 80992-1-RR (same clone as 80992-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ubiquitin antibody (80992-1-RR, Clone: 6H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80992-1-RR

    Affinity and Kinetic Characterization of 80992-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80992-1-RR against Human ubiquitin were performed. The affinity constant is 1.57 nM.

ubiquitin Recombinant antibody, PBS Only

ubiquitin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80992-1-PBS

Print datasheet

CloneNo. 6H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster, yeast, dog, spinach

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy UBB Ready-To-Use IHC Kit. UBB primary antibody included.

产品信息

80992-1-PBS targets ubiquitin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster, yeast, dog, spinach samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, hamster, yeast, dog, spinach
Immunogen ubiquitin fusion protein Ag0260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin B
Synonyms ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6
GenBank Accession NumberBC000379
Gene Symbol ubiquitin
Gene ID (NCBI) 7314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0CG47
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.