• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80992-1-RR (same clone as 80992-1-PBS)

    WB analysis using 80992-1-RR (same clone as 80992-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80992-1-RR (ubiquitin antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 80992-1-RR (same clone as 80992-1-PBS)

    IHC staining of human pancreas cancer using 80992-1-RR (same clone as 80992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBB antibody included
  • IHC staining of human lung cancer using 80992-1-RR (same clone as 80992-1-PBS)

    IHC staining of human lung cancer using 80992-1-RR (same clone as 80992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 80992-1-RR (ubiquitin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with UBB antibody included
  • IF Staining of HeLa using 80992-1-RR (same clone as 80992-1-PBS)

    IF Staining of HeLa using 80992-1-RR (same clone as 80992-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ubiquitin antibody (80992-1-RR, Clone: 6H6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 80992-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80992-1-RR

    Affinity and Kinetic Characterization of 80992-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80992-1-RR against Human ubiquitin were performed. The affinity constant is 1.57 nM.

ubiquitin Recombinant antibody, PBS Only

ubiquitin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 80992-1-PBS

产品说明书

CloneNo. 6H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, hamster, yeast, dog, spinach

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy UBB Ready-To-Use IHC Kit. UBB primary antibody included.

产品信息

80992-1-PBS targets ubiquitin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, hamster, yeast, dog, spinach samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, hamster, yeast, dog, spinach
免疫原 ubiquitin fusion protein Ag0260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ubiquitin B
别名 ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6
GenBank蛋白编号BC000379
基因名称 ubiquitin
Gene ID (NCBI) 7314
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP0CG47
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.