ubiquitin Recombinant antibody
ubiquitin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, hamster, yeast, dog, spinach
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
6H6
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells, MG132 treated NIH/3T3 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:10000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IP | See 3 publications below |
产品信息
80992-1-RR targets ubiquitin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, hamster, yeast, dog, spinach samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IP |
Tested Reactivity | human, mouse, rat, hamster, yeast, dog, spinach |
Cited Reactivity | human, mouse |
Immunogen | ubiquitin fusion protein Ag0260 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ubiquitin B |
Synonyms | ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6 |
GenBank Accession Number | BC000379 |
Gene Symbol | ubiquitin |
Gene ID (NCBI) | 7314 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P0CG47 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ubiquitin antibody 80992-1-RR | Download protocol |
IHC protocol for ubiquitin antibody 80992-1-RR | Download protocol |
IF protocol for ubiquitin antibody 80992-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Signal Repurposing a plant alkaloid homoharringtonine targets insulinoma associated-1 in N-Myc-activated neuroblastoma | ||
Clin Transl Oncol LncRNA XXYLT1-AS2 promotes tumor progression via autophagy inhibition through ubiquitinated degradation of TFEB in hepatocellular carcinoma | ||
Free Radic Biol Med Carnitine functions as an enhancer of NRF2 to inhibit osteoclastogenesis via regulating macrophage polarization in osteoporosis | ||
iScience CircEZH2 promotes gallbladder cancer progression and lipid metabolism reprogramming through the miR-556-5p/SCD1 axis | ||