ubiquitin Recombinant antibody

ubiquitin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, hamster, yeast, dog, spinach

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

6H6

Cat no : 80992-1-RR

Print datasheet

Synonyms

ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6



经过测试的应用

Positive WB detected inNIH/3T3 cells, MG132 treated NIH/3T3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy UBB Ready-To-Use IHC Kit. UBB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80992-1-RR targets ubiquitin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, hamster, yeast, dog, spinach samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat, hamster, yeast, dog, spinach
Cited Reactivityhuman, mouse
Immunogen ubiquitin fusion protein Ag0260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ubiquitin B
Synonyms ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B, 6H6
GenBank Accession NumberBC000379
Gene Symbol ubiquitin
Gene ID (NCBI) 7314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP0CG47
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

实验方案

Product Specific Protocols
WB protocol for ubiquitin antibody 80992-1-RRDownload protocol
IHC protocol for ubiquitin antibody 80992-1-RRDownload protocol
IF protocol for ubiquitin antibody 80992-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

Repurposing a plant alkaloid homoharringtonine targets insulinoma associated-1 in N-Myc-activated neuroblastoma

Authors - Chiachen Chen
humanIP

Clin Transl Oncol

LncRNA XXYLT1-AS2 promotes tumor progression via autophagy inhibition through ubiquitinated degradation of TFEB in hepatocellular carcinoma

Authors - Xuejie Li
mouseWB,IP

Free Radic Biol Med

Carnitine functions as an enhancer of NRF2 to inhibit osteoclastogenesis via regulating macrophage polarization in osteoporosis

Authors - Tao Yang
mouseWB,IP

Biochem Pharmacol

BCL6 confers resistance to HDAC inhibitors in DLBCL

Authors - Gao Fan
humanWB

iScience

CircEZH2 promotes gallbladder cancer progression and lipid metabolism reprogramming through the miR-556-5p/SCD1 axis

Authors - Huanjun Tong
humanWB

J Med Chem

Discovery of Small-Molecule Degraders for Alpha-Synuclein Aggregates

Authors - Yichen Tong