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Calpastatin Monoclonal antibody, PBS Only (Capture)

Calpastatin Monoclonal Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G12A2

Cat no : 67107-1-PBS

Print datasheet

Synonyms

CAST, Sperm BS-17 component, Calpain inhibitor, 1G12A2

验证数据展示

  • WB analysis of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    WB analysis of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67107-1-Ig (CAST antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    IF Staining of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Calpastatin antibody (67107-1-Ig, Clone: 1G12A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    FC experiment of HeLa using 67107-1-Ig (same clone as 67107-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Calpastatin Monoclonal antibody (67107-1-Ig, Clone:1G12A2) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67107-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50744-2

    Cytometric bead array standard curve of MP50744-2

    Cytometric bead array standard curve of MP50744-2, CAST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67107-1-PBS. Detection antibody: 67107-3-PBS. Standard:Ag28669. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50744-3

    Cytometric bead array standard curve of MP50744-3

    Cytometric bead array standard curve of MP50744-3, CAST Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67107-1-PBS. Detection antibody: 67107-4-PBS. Standard:Ag28669. Range: 0.098-100 ng/mL.

产品信息

67107-1-PBS targets Calpastatin as part of a matched antibody pair:

MP50744-2: 67107-1-PBS capture and 67107-3-PBS detection (validated in Cytometric bead array)

MP50744-3: 67107-1-PBS capture and 67107-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Calpastatin fusion protein Ag28669 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calpastatin
Synonyms CAST, Sperm BS-17 component, Calpain inhibitor, 1G12A2
Calculated Molecular Weight 667 aa, 72 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC013579
Gene Symbol Calpastatin
Gene ID (NCBI) 831
RRIDAB_2882411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20810
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C