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Mouse CD45 Recombinant antibody, PBS Only (Capture)

CD45 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240920B4

Cat no : 83396-14-PBS

Print datasheet

Synonyms

Ptprc, Leukocyte common antigen, LCA, B220, 240920B4

验证数据展示

  • Sandwich ELISA standard curve of MP00412-4

    Sandwich ELISA standard curve of MP00412-4

    Sandwich ELISA standard curve of MP00412-4, Mouse CD45 Recombinant Matched Antibody Pair - PBS only. 83396-14-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0845. 83396-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sample test of MP00412-4

    Sample test of MP00412-4

    The mean mouse CD45 concentration was determined to be 39.3 ng/mL in RAW 264.7 cell extract based on a 4.8 mg/mL extract load.

  • Sample test of MP00412-4

    Sample test of MP00412-4

    RAW 264.7 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD45, and measured 154.9 pg/mL.

产品信息

83396-14-PBS targets CD45 as part of a matched antibody pair:

MP00412-4: 83396-14-PBS capture and 83396-7-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Ptprc, Leukocyte common antigen, LCA, B220, 240920B4
Calculated Molecular Weight145 kDa
GenBank Accession NumberNM_001111316.2
Gene Symbol CD45
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06800-4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C