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Mouse NCAM1/CD56 Recombinant antibody, PBS Only (Capture)

NCAM1/CD56 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240936B9

Cat no : 83883-2-PBS

Print datasheet

Synonyms

Ncam1, CD56, 240936B9, NCAM 1, Ncam

验证数据展示

  • WB analysis of C6 using 83883-2-RR (same clone as 83883-2-PBS)

    WB analysis of C6 using 83883-2-RR (same clone as 83883-2-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 83883-2-RR (Ncam1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83883-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00841-1

    Cytometric bead array standard curve of MP00841-1

    Cytometric bead array standard curve of MP00841-1, MOUSE NCAM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83883-2-PBS. Detection antibody: 83883-4-PBS. Standard: Eg0652. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 83883-2-RR

    Affinity and Kinetic Characterization of 83883-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83883-2-RR against Mouse Ncam1 were performed. The affinity constant is 0.833 nM.

产品信息

83883-2-PBS targets NCAM1/CD56 as part of a matched antibody pair:

MP00841-1: 83883-2-PBS capture and 83883-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity rat
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name neural cell adhesion molecule 1
Synonyms Ncam1, CD56, 240936B9, NCAM 1, Ncam
Calculated Molecular Weight119 kDa
Observed Molecular Weight140 kDa
Gene Symbol Ncam1
Gene ID (NCBI) 17967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13595-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily. It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis. NCAM1 is expressed on human neurons, glial cells, skeletal muscle cells, NK cells, and a subset of T cells, and the expression is observed in a wide variety of human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas. Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kDa, are generated by alternative splicing of mRNA (PMID: 9696812). The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII). All three forms can be posttranslationally modified by the addition of polysialic acid (PSA) (PMID: 14976519). Several other isoforms have also been described (PMID: 1856291).