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TRAIL Recombinant antibody, PBS Only (Detector)

TRAIL Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241419C12

Cat no : 84280-1-PBS

Print datasheet

Synonyms

TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253

验证数据展示

  • WB analysis using 84280-1-RR (same clone as 84280-1-PBS)

    WB analysis using 84280-1-RR (same clone as 84280-1-PBS)

    Human milk samples were subjected to SDS PAGE followed by western blot with 84280-1-RR (TRAIL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84280-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01166-1

    Cytometric bead array standard curve of MP01166-1

    Cytometric bead array standard curve of MP01166-1, TRAIL Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84280-2-PBS. Detection antibody: 84280-1-PBS. Standard: RP02339. Range: 0.313-40 ng/mL.

产品信息

84280-1-PBS targets TRAIL as part of a matched antibody pair:

MP01166-1: 84280-2-PBS capture and 84280-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 10
Synonyms TNFSF10, TNFSF 10, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253
Calculated Molecular Weight 281 aa, 33 kDa
Observed Molecular Weight22-25 kDa
GenBank Accession NumberBC032722
Gene Symbol TRAIL
Gene ID (NCBI) 8743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50591
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TRAIL, also known as CD253 or TNFSF10 (tumor necrosis factor superfamily member 10), is a typical death ligand expressed on natural killer cells and cytotoxic T lymphocytes. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL induces apoptotic cell death in cancer by binding to its functional death receptors, death receptor (DR) 4 (TNFRSF10A/TRAIL-R1) and DR5 (TNFRSF10B/TRAIL-R2) to activate the extrinsic apoptosis pathway. TRAIL also activates c-Jun N-terminal kinase (MAPK8/JNK) and the transcription factor nuclear factor-κB (NFκB). The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.