1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IGF2R-Specific (CL488-20253) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human IGF2R-Specific (CL647-20253) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug PE Anti-Mouse IFN gamma (PE-65153, Clone: XMG1.2).
Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL488-60207 (Galectin-3 antibody) at dilution of 1:50.
Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using CL488-60207 (Galectin-3 antibody) at dilution of 1:50.
1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Galectin-3 (CL488-60207, Clone:1C1B2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IFN-γ primed Balb/c mouse peritoneal macrophages stimulated with LPS in the presence of a protein transport inhibitor were treated with fixation and permeabilization reagents and intracellularly stained with CoraLite® Plus 488-conjugated Rat IgG2a Isotype Control (left) or 5 ul CoraLite® Plus 488 Anti-Mouse IL-12/IL-23 p40 (CL488-65097, Clone: C17.8) (right).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66020 (Bcl-XL antibody) at dilution of 1:100.
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Bcl-XL (CL488-66020, Clone:4C12A6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 PC-3 cells were stained with 0.5 ug CoraLite®488-conjugated Noggin antibody (CL488-69010, Clone: 2A11C1) (green), or 0.5 ug isotype control (black). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Osteopontin (CL488-22952) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
