SDS-PAGE Gel Recipes
SDS-PAGE Gel Recipes
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In order to target proteins with MWs between 20 and 200 kDa, you will need to create a conventional SDS-PAGE gel using the recipes shown below. The percentage of gel you require corresponds with the MW of your target protein.
Recipe 1
Recipe 2
| 2x Separating Buffer Recipe (makes 1000ml) | |
| Tris HCl (pH 8.8) | 90.8g |
| SDS | 2.0g |
| Dissolve compounds thoroughly. Adjust pH slowly to pH 8.8 with concentrated HCl, then add ddH2O to 1000ml. | |
Recipe 3
| 2x Stacking Buffer Recipe (makes 1000ml) | |
| Tris HCl (pH 6.8) | 30.35g |
| SDS | 2.0g |
| Dissolve compounds thoroughly. Adjust pH slowly to pH 6.8 with concentrated HCl, then add ddH2O to 1000ml. | |
Recipe 4
| 1x Running Buffer Recipe (makes 1000ml) | |
| Tris-base | 1.51g |
| Glycine | 7.5g |
| SDS | 0.5g |
| Dissolve compounds thoroughly, then add ddH2O to 1000ml. | |
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