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Tricine Gel Recipe For Low Molecular Weight Proteins

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For target proteins with MWs of less than 20 kDa, a tricine gel system will obtain higher resolution and is highly recommended. Make three layers of tricine gels as laid out in the following table and diagram. Apply specific tricine gel running buffer to the running system and perform transfer as usual.

Recipe 1
Items Stacking Intermediate Separation
Gel Percentage 4% 10% 15%
Gel Volume 2ml 3ml 6ml
38% Glycerol - - 1.6
ddH20 1.4 1.2 -
30% Acrylamide 0.3 0.8 2.7
3.0 M Tris HCl (pH 8.5) - 1 2.14
1.0 M Tris HCl (pH 6.8) 0.3 - -
10% SDS 0.02 0.03 0.06
10% APS 0.02 0.03 0.06
TEMED 0.002 0.003 0.003