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IP Troubleshooting

Immunoprecipitation (IP) Troubleshooting

Immunoprecipitation (IP) troubleshooting guide by ChromoTek (part of Proteintech). This video aims to address the common issues when running IP experiments:

1. Recommended controls forgotten
2. High background in B fraction
3. No pulldown of GFP-POI
4. No GFP-POI can be detected
5. Low amount of GFP-POI eluted with SDS-sample buffer