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Perilipin-2 Polyclonal antibody

Perilipin-2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15294-1-AP

Print datasheet

Synonyms

ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein



经过测试的应用

Positive WB detected inmouse liver tissue, K-562 cells, rat liver tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human renal cell carcinoma tissue, human liver tissue, human colon cancer tissue, human prostate cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inoleic acid treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15294-1-AP targets Perilipin-2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, canine, monkey, zebrafish, bovine
Immunogen Perilipin-2 fusion protein Ag7539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adipose differentiation-related protein
Synonyms ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC005127
Gene Symbol Perilipin-2
Gene ID (NCBI) 123
RRIDAB_2878122
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99541
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

实验方案

Product Specific Protocols
WB protocol for Perilipin-2 antibody 15294-1-APDownload protocol
IHC protocol for Perilipin-2 antibody 15294-1-APDownload protocol
IF protocol for Perilipin-2 antibody 15294-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nature

iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer

Authors - Dong Shin Park
humanIF

Nature

The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle.

Authors - Simona Ricciardi
mouse,humanIF,IHC

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
mouseIF

Nat Commun

Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy

Authors - Donghai Cui
mouseWB

Mol Cell

Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.

Authors - Anyuan He
mouseWB

Nat Commun

O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.

Authors - Yunfan Yang