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验证数据展示

  • IF Staining of HeLa using CL488-15294

    IF Staining of HeLa using CL488-15294

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP/Perilipin 2 antibody (CL488-15294) at dilution of 1:400, CL594-Phalloidine (red).

  • IF Staining of HeLa using CL488-15294

    IF Staining of HeLa using CL488-15294

    Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using CoraLite® Plus 488 ADRP/Perilipin 2 antibody (CL488-15294) at dilution of 1:400.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Perilipin-2 Polyclonal antibody

Perilipin-2 Polyclonal Antibody for IF/ICC
Cat No. CL488-15294

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inoleic acid treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ADRP Ready-To-Use IHC Kit. ADRP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15294-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15294 targets Perilipin-2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Perilipin-2 fusion protein Ag7539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adipose differentiation-related protein
Synonyms ADFP, Perilipin 2, ADRP/Perilipin 2, ADRP, Adipose differentiation-related protein
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC005127
Gene Symbol Perilipin-2
Gene ID (NCBI) 123
RRIDAB_2919116
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99541
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Perilipin-2 antibody CL488-15294Download protocol
Standard Protocols
Click here to view our Standard Protocols