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ADRP/Perilipin-2 Polyclonal antibody
ADRP/Perilipin 2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (5)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
109
验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, K-562 cells, rat liver tissue, NIH/3T3 cells |
Positive IHC detected in | human liver cancer tissue, mouse liver tissue, human liver tissue, human renal cell carcinoma tissue, human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | oleic acid treated HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:8000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15294-1-AP targets ADRP/Perilipin 2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, monkey, zebrafish, pig, canine, bovine |
Immunogen | ADRP/Perilipin 2 fusion protein Ag7539 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | adipose differentiation-related protein |
Synonyms | ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, PLIN2 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 45-48 kDa |
GenBank Accession Number | BC005127 |
Gene Symbol | ADFP |
Gene ID (NCBI) | 123 |
RRID | AB_2878122 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q99541 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ADRP/Perilipin 2 antibody 15294-1-AP | Download protocol |
IHC protocol for ADRP/Perilipin 2 antibody 15294-1-AP | Download protocol |
IF protocol for ADRP/Perilipin 2 antibody 15294-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature iPS-cell-derived microglia promote brain organoid maturation via cholesterol transfer | ||
Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. | ||
Nat Commun Spliceosome component Usp39 contributes to hepatic lipid homeostasis through the regulation of autophagy | ||
Mol Cell Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation. | ||
Nat Commun O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. |