验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66773-1-Ig targets Apolipoprotein A II/APOA2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Apolipoprotein A II/APOA2 fusion protein Ag9863 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | apolipoprotein A-II |
| Synonyms | APOA2, apolipoprotein A II, ApoA-II, Apo-AII, ApoA II |
| Calculated Molecular Weight | 100 aa, 11 kDa |
| Observed Molecular Weight | 8-11 kDa |
| GenBank Accession Number | BC005282 |
| Gene Symbol | APOA2 |
| Gene ID (NCBI) | 336 |
| RRID | AB_2882119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02652 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apolipoprotein A-II (APOA2) is a major component of high density lipoprotein (HDL) and it plays an important role in directing the fate of lipid metabolism among HDL. It is primarily synthesized by liver. The predicted MW of ApoA2 is 11 kDa, while the mature form is smaller (7-10 kDa) since the signal peptide was cleaved. In humans, most of circulating apoA2 exist as dimer. Five types of APOA2 dimer exist: homodimer apoA2-ATQ/ATQ, heterodimer apoA2-ATQ/AT, homodimer apoA2-AT/AT, apoA2-AT/A, and apoA2-A/A. ApoA2 isoforms are considered to be some of the most promising serum/plasma biomarkers for aiding the early detection of pancreatic cancer. (PMID: 29081441, 29481802)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Apolipoprotein A II/APOA2 antibody 66773-1-Ig | Download protocol |
| IHC protocol for Apolipoprotein A II/APOA2 antibody 66773-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |