验证数据展示
经过测试的应用
| Positive WB detected in | HuH-7 cells, L02 cells, HepG2 cells, pig liver tissue, mouse hepatocytes, HSC-T6 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66692-1-Ig targets ASGR1 in WB, IHC, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, Blocking assay |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Immunogen | ASGR1 fusion protein Ag2310 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | asialoglycoprotein receptor 1 |
| Synonyms | asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1 |
| Calculated Molecular Weight | 291 aa, 33 kDa |
| Observed Molecular Weight | 42-46 kDa |
| GenBank Accession Number | BC032130 |
| Gene Symbol | ASGR1 |
| Gene ID (NCBI) | 432 |
| RRID | AB_2882046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P07306 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASGR1 antibody 66692-1-Ig | Download protocol |
| IHC protocol for ASGR1 antibody 66692-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
