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  • Featured Product
  • KD/KO Validated

ASGR1 Monoclonal antibody, PBS Only

ASGR1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5F5

Cat no : 66692-1-PBS

Print datasheet

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

验证数据展示

  • WB analysis of HepG2 using 66692-1-Ig (same clone as 66692-1-PBS)

    WB analysis of HepG2 using 66692-1-Ig (same clone as 66692-1-PBS)

    WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • WB analysis using 66692-1-Ig (same clone as 66692-1-PBS)

    WB analysis using 66692-1-Ig (same clone as 66692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

    IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

    IHC staining of human liver using 66692-1-Ig (same clone as 66692-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IF Staining of mouse liver using 66692-1-Ig (same clone as 66692-1-PBS)

    IF Staining of mouse liver using 66692-1-Ig (same clone as 66692-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using ASGR1 antibody (66692-1-Ig, Clone: 1G5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66692-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.

产品信息

66692-1-PBS targets ASGR1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ASGR1 fusion protein Ag2310 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight 42-46 kDa
GenBank Accession NumberBC032130
Gene Symbol ASGR1
Gene ID (NCBI) 432
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07306
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.