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ASGR1 Monoclonal antibody

ASGR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G5F5

Cat no : 66692-1-Ig

Print datasheet

Synonyms

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

验证数据展示

  • WB analysis of HepG2 using 66692-1-Ig

    WB analysis of HepG2 using 66692-1-Ig

    WB result of ASGR1 antibody (66692-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ASGR1 transfected HepG2 cells.

  • WB analysis using 66692-1-Ig

    WB analysis using 66692-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66692-1-Ig (ASGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66692-1-Ig

    IHC staining of human liver using 66692-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IHC staining of human liver using 66692-1-Ig

    IHC staining of human liver using 66692-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66692-1-Ig (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IF Staining of mouse liver using 66692-1-Ig

    IF Staining of mouse liver using 66692-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using ASGR1 antibody (66692-1-Ig, Clone: 1G5F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHuH-7 cells, L02 cells, HepG2 cells, pig liver tissue, mouse hepatocytes, HSC-T6 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66692-1-Ig targets ASGR1 in WB, IHC, IF-P, ELISA, Blocking assay applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, Blocking assay
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Immunogen ASGR1 fusion protein Ag2310 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight 42-46 kDa
GenBank Accession NumberBC032130
Gene Symbol ASGR1
Gene ID (NCBI) 432
RRIDAB_2882046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP07306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.

实验方案

Product Specific Protocols
WB protocol for ASGR1 antibody 66692-1-IgDownload protocol
IHC protocol for ASGR1 antibody 66692-1-IgDownload protocol
IF protocol for ASGR1 antibody 66692-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,Blocking assay

J Med Virol

The use of human iPSC-derived alveolar organoids to explore SARS-CoV-2 variant infections and host responses

Authors - Chaitanya Gandikota
View Article