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ATF4 Polyclonal antibody 210 Publications

Rabbit Polyclonal| Catalog number: 10835-1-AP

Featured Product KD/KO validated

浓度: 1000 μg/ml

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Applications

Tested Applications:
IF, IHC, IP, WB, ELISA
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Cited Applications:
ChIP, FC, IF, IHC, IP, WB


Images See all

IHC staining of human breast cancer using 10835-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IF Staining of HepG2 using 10835-1-AP

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10835-1-AP (ATF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

WB analysis of A431 using 10835-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IP experiment of HEK-293 using 10835-1-AP

IP Result of anti-ATF4 (IP:10835-1-AP, 3ug; Detection:10835-1-AP 1:400) with HEK-293 cells lysate 6000ug.

Species Specificity:
human, mouse, rat
Cited Species:
hamster, human, mouse, pig, rat, zebrafish
Positive WB detected in:
A431 cells, mouse kidney tissue, rat brain tissue, HEK-293 cells
Positive IP detected in:
HEK-293 cells
Positive IHC detected in:
human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ATF4 fusion protein Ag1279
Full name:
activating transcription factor 4 (tax-responsive enhancer element B67)
Calculated molecular weight:
39 kDa
Observed molecular weight:
45-50 kDa
Uniprot:
P18848
Gene ID (NCBI):
468
Gene symbol
ATF4
Synonyms
ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB

Background

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 45-50 kDa ATF4 protein and its phosphorylated forms (50 kDa). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

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