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FITC-conjugated ATF4 Polyclonal antibody
ATF4 Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, FC (Intra) and More (1)
Conjugate
FITC Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells, Tunicamycin treated HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.60 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
FITC-10835 targets ATF4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | ATF4 fusion protein Ag1279 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | activating transcription factor 4 (tax-responsive enhancer element B67) |
Synonyms | TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4 |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | BC022088 |
Gene Symbol | ATF4 |
Gene ID (NCBI) | 468 |
RRID | AB_2883737 |
Conjugate | FITC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 498 nm / 526 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P18848 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IF protocol for FITC ATF4 antibody FITC-10835 | Download protocol |
FC protocol for FITC ATF4 antibody FITC-10835 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Cell Int MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin. | ||
Int J Nanomedicine Nanoparticle/Engineered Bacteria Based Triple-Strategy Delivery System for Enhanced Hepatocellular Carcinoma Cancer Therapy |