FITC-conjugated ATF4 Polyclonal antibody

ATF4 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra), IF

Conjugate

FITC Fluorescent Dye

Cat No : FITC-10835

Print datasheet

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, FITC-10835, TAXREB67, TXREB



经过测试的应用

Positive IF detected inHepG2 cells, Tunicamycin treated HeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

FITC-10835 targets ATF4 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ATF4 fusion protein Ag1279 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Synonyms ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, FITC-10835, TAXREB67, TXREB
Calculated Molecular Weight 39 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC022088
Gene Symbol ATF4
Gene ID (NCBI) 468
RRIDAB_2883737
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths498 nm / 526 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18848
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38kd ATF4 protein and its phosphorylated forms (50kd). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

实验方案

Product Specific Protocols
IF protocol for FITC ATF4 antibody FITC-10835Download protocol
FC protocol for FITC ATF4 antibody FITC-10835Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Cell Int

MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin.

Authors - Yibo Dong