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验证数据展示

  • IHC staining of human breast cancer using 10835-1-AP (same clone as 10835-1-PBS)

    IHC staining of human breast cancer using 10835-1-AP (same clone as 10835-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATF4 antibody included
  • WB analysis of A431 using 10835-1-AP (same clone as 10835-1-PBS)

    WB analysis of A431 using 10835-1-AP (same clone as 10835-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10835-1-AP (same clone as 10835-1-PBS)

    IP experiment of HEK-293 using 10835-1-AP (same clone as 10835-1-PBS)

    IP result of anti-ATF4 (IP:10835-1-AP, 3ug; Detection:10835-1-AP 1:400) with HEK-293 cells lysate 6000ug. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 10835-1-AP (same clone as 10835-1-PBS)

    IHC staining of mouse colon using 10835-1-AP (same clone as 10835-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ATF4 antibody included
  • IF Staining of HeLa using 10835-1-AP (same clone as 10835-1-PBS)

    IF Staining of HeLa using 10835-1-AP (same clone as 10835-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using ATF4 antibody (10835-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10835-1-AP (same clone as 10835-1-PBS)

    FC experiment of HeLa using 10835-1-AP (same clone as 10835-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human ATF4 (10835-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 10835-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ATF4 Polyclonal antibody, PBS Only

ATF4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10835-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.

产品信息

10835-1-PBS targets ATF4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ATF4 fusion protein Ag1279 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activating transcription factor 4 (tax-responsive enhancer element B67)
别名 TXREB, Tax-responsive enhancer element-binding protein 67, Cyclic AMP-responsive element-binding protein 2, CREB2, cAMP-dependent transcription factor ATF-4
计算分子量 39 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC022088
基因名称 ATF4
Gene ID (NCBI) 468
RRIDAB_2058600
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18848
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

What is the molecular weight of ATF4?

The molecular weight of ATF is 38.6 kD.

 

What is ATF4?

Activating transcription factor 4 (ATF4), also known as cAMP-response element-binding protein 2 (CREB2), is a substrate of RSK2 and a basic leucine-zipper transcription factor (PMIDs: 16000305, 17485283).

 

What the function of ATF4?

ATF4 its a transcription factor that controls the transcriptional activity of mature osteoblasts. ATF4 is particularly critical for their timely onset and terminal differentiation, as well as expression of Bsp and osteocalcin. Knockout animals displayed reduction or delay in bone mineralization and have severely reduced bone volume. ATF4 is also part of the PERK-eIF2α-ATF4-CHOP apoptosis pathway, which is activated by ER stress, and it likely plays a role related to tumor cell survival (PMIDs: 18083928, 16000305, 30134550).

 

What is the effect of ATF4 interaction with RSK2?

ATF4 and RSK2 posttranscriptionally regulate type I collagen synthesis. Lack of RSK2 phosphorylation of AFT4 may contribute to skeletal phenotypes associated with Coffin-Lowry Syndrome (PMID: 17485283).

 

Where is ATF4 expressed?

ATF4 protein is predominantly expressed in osteoblasts, although its corresponding Atf4 mRNA is ubiquitously expressed (PMID: 16000305).

 

What regulates ATF4 expression?

ATF4 is regulated by a ubiquitin/proteasomal pathway, which is less active in osteoblasts by inhibition with MG115 (PMID: 16000305).

 

How does ATF4 expression affect Ocn mRNA?

Inhibition of the degradation pathway leads to ATF4 accumulation and induces Ocn mRNA expression in non-osteoblastic cells (PMID: 16000305).

 

Does ATF4 have the ability to induce osteoblast-specific gene expression even in non-osteoblastic cells?

Yes, ATF4, as well as other osteoblast differentiation factors, has this ability. AFT4 interactions with Runx2 can stimulate osteoblast-specific osteocalcin gene expression. (PMIDs: 16000305, 17485283)