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GABARAPL1 Polyclonal antibody

GABARAPL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11010-1-AP

Print datasheet

Synonyms

APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1



经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, rat brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inChloroquine treated HeLa cells, Chloroquine treated HepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11010-1-AP targets GABARAPL1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, prawn
Immunogen GABARAPL1 fusion protein Ag1473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GABA(A) receptor-associated protein like 1
Synonyms APG8 LIKE, APG8L, ATG8, ATG8L, GABARAPL1, GEC 1, GEC1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC009309
Gene Symbol GABARAPL1
Gene ID (NCBI) 23710
RRIDAB_2294415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0R8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABARAPL1 (GABARAP-like protein 1), also named ATG8, GEC1, APG8L, ATG8L, and APG8-LIKE, is a member of the GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as an estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2 and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta, and skeletal muscle and at very low levels in the thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.

实验方案

Product Specific Protocols
WB protocol for GABARAPL1 antibody 11010-1-APDownload protocol
IHC protocol for GABARAPL1 antibody 11010-1-APDownload protocol
IF protocol for GABARAPL1 antibody 11010-1-APDownload protocol
IP protocol for GABARAPL1 antibody 11010-1-APDownload protocol
FC protocol for GABARAPL1 antibody 11010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Circulation

Markers of autophagy are downregulated in failing human heart after mechanical unloading.

Authors - Kassiotis Christos C
humanWB,IF,IP

J Extracell Vesicles

Secretion of pro-angiogenic extracellular vesicles during hypoxia is dependent on the autophagy-related protein GABARAPL1.

Authors - Tom G Keulers
mouseWB

Cell Metab

FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells.

Authors - Zhao Jinghui J
mouseWB

Autophagy

Analysis of ATG4C function in vivo

Authors - Isaac Tamargo-Gómez
mouseWB,IF

J Clin Invest

Autophagy is essential for mouse sense of balance.

Authors - Mari?o Guillermo G
humanWB

Autophagy

GABARAPL1 antibodies: target one protein, get one free!

Authors - Le Grand Jaclyn Nicole JN