验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Chloroquine treated HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-11010 targets GABARAPL1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | GABARAPL1 fusion protein Ag1473 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GABA(A) receptor-associated protein like 1 |
| 别名 | GABA(A) receptor-associated protein-like 1, Early estrogen-regulated protein, ATG8L, ATG8, APG8L |
| 计算分子量 | 14 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC009309 |
| 基因名称 | GABARAPL1 |
| Gene ID (NCBI) | 23710 |
| RRID | AB_2934401 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity Purified |
| UNIPROT ID | Q9H0R8 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GABARAPL1 (GABARAP-like protein 1), also named as ATG8, GEC1, APG8L, ATG8L and APG8-LIKE, is a member of GABARP (GABAA receptor-associated protein) family. GABARAPL1 was initially identified as estrogen-regulated gene and the protein acts in receptor and vesicle transport. It's also involved in the process of autophagy like GABARAP and GABARAPL2, and may be considered as an autophagic marker. It is expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle and at very low levels in thymus and small intestine. The antibody is specific to GABARAPL1, and it doesn't recognize the recombinant GABARAP and GABARAPL2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GABARAPL1 antibody CL488-11010 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
