验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, Jurkat cells, HEK-293 cells, pig brain tissue, mouse brain tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
67057-1-Ig targets ATX3,ATXN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, mouse, pig |
| Cited Reactivity | human, mouse |
| Immunogen | ATX3,ATXN3 fusion protein Ag6627 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ataxin 3 |
| Synonyms | AT3, ataxin 3, ATX3, ATX3,ATXN3, ATXN3, JOS, MJD, MJD1, SCA3 |
| Calculated Molecular Weight | 370 aa, 43 kDa |
| Observed Molecular Weight | 41-45 kDa |
| GenBank Accession Number | BC033711 |
| Gene Symbol | ATXN3 |
| Gene ID (NCBI) | 4287 |
| RRID | AB_2882368 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P54252 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATXN3, which has deubiquitinase activity and act as a component of the ubiquitin proteasome system, plays a role in transcriptional regulation and neuroprotection. ATXN3 interacts with RAD23, HHR23A and HHR23B, involves in the pathology of MJD. ATXN3 is a mixed-linkage, chain-editing enzyme and that the UIM region of ATXN3 regulates its substrate specificity. Contains an N-terminal deubiquitinating domain, called the Josephin domain, followed by 2 ubiquitin-interacting motifs (UIMs) and a polyQ tract near the C terminus. ATXN3 can be phosphorylated in a protein casein kinase-2-dependent manner, thus the MW would be larger than the predicted one.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ATX3,ATXN3 antibody 67057-1-Ig | Download protocol |
| IHC protocol for ATX3,ATXN3 antibody 67057-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest CRISPR screening identifies the deubiquitylase ATXN3 as a PD-L1-positive regulator for tumor immune evasion | ||
J Biol Chem ATXN3 functions as a tumor suppresser through potentiating Galectin-9-mediated apoptosis in human colon adenocarcinoma |
