验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, rabbit serum |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| ELISA | See 1 publications below |
产品信息
66634-1-Ig targets C5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | WB, ELISA |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | C5 fusion protein Ag17882 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | complement component 5 |
| Synonyms | C5, C5b, C5, C5a, Complement C5, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 |
| Calculated Molecular Weight | 1676 aa, 188 kDa |
| Observed Molecular Weight | 115 kDa |
| GenBank Accession Number | BC113740 |
| Gene Symbol | C5 |
| Gene ID (NCBI) | 727 |
| RRID | AB_2881993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01031 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The complement system is an important effector that bridges the innate and adaptive immune systems. The fifth component of complement, C5, is part of the complement cascade and plays an important role in inflammation and cell killing. It consists of disulfide-linked alpha and beta polypeptide chains but can be cleaved into two active peptides (C5a and C5b) by C5 convertases. Mutations and defects in C5 are associated with severe recurrent infections. (PMID: 20010915; PMID: 8011297; PMID: 6554279)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for C5 antibody 66634-1-Ig | Download protocol |
| IF protocol for C5 antibody 66634-1-Ig | Download protocol |
| FC protocol for C5 antibody 66634-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Proteomics Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer. | ||
Virulence Streptococcus suis subtilisin-like serine proteases SspA-1 and SspA-2 interplay with complement C3a and C5a to facilitate bacterial immune evasion and infection |
