• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD146/MCAM Monoclonal antibody

CD146/MCAM Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4D8A9

Cat no : 66153-1-Ig

Print datasheet

Synonyms

CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32

验证数据展示

  • WB analysis of A375 using 66153-1-Ig

    WB analysis of A375 using 66153-1-Ig

    A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66153-1-Ig

    WB analysis of HepG2 using 66153-1-Ig

    Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • IHC staining of human liver cancer using 66153-1-Ig

    IHC staining of human liver cancer using 66153-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD146/MCAM antibody included
  • IHC staining of human liver cancer using 66153-1-Ig

    IHC staining of human liver cancer using 66153-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD146/MCAM antibody included
  • IF Staining of human liver cancer using 66153-1-Ig

    IF Staining of human liver cancer using 66153-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 66153-1-Ig

    IF Staining of human liver cancer using 66153-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HUVEC using 66153-1-Ig

    IF Staining of HUVEC using 66153-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, A375 cells, L02 cells, HUVEC cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue, HUVEC cells
Positive IF/ICC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD146/MCAM Ready-To-Use IHC Kit. CD146/MCAM primary antibody included.
Planning an IF experiment? We recommend our CoraLite®555 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66153-1-Ig targets CD146/MCAM in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD146/MCAM fusion protein Ag11855 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name melanoma cell adhesion molecule
Synonyms CD146, 4D8A9, MUC18, Melanoma-associated antigen MUC18, Melanoma-associated antigen A32
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC056418
Gene Symbol CD146
Gene ID (NCBI) 4162
RRIDAB_2881549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.

实验方案

Product Specific Protocols
WB protocol for CD146/MCAM antibody 66153-1-IgDownload protocol
IHC protocol for CD146/MCAM antibody 66153-1-IgDownload protocol
IF protocol for CD146/MCAM antibody 66153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanFC

Exp Cell Res

Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.

Authors - Xun Xi
View Article
humanWB

Cell Signal

Glioma-derived small extracellular vesicles induce pericyte-phenotype transition of glioma stem cells under hypoxic conditions

Authors - Yue Cheng
View Article