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CD43 Monoclonal antibody

CD43 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A11D6

Cat no : 66224-1-Ig

Print datasheet

Synonyms

Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein, CD43 cytoplasmic tail

验证数据展示

  • WB analysis of Jurkat using 66224-1-Ig

    WB analysis of Jurkat using 66224-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66224-1-Ig

    IHC staining of human tonsillitis using 66224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human tonsillitis using 66224-1-Ig

    IHC staining of human tonsillitis using 66224-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD43 antibody included
  • IF Staining of human tonsillitis using 66224-1-Ig

    IF Staining of human tonsillitis using 66224-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66224-1-Ig (CD43 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of Jurkat using 66224-1-Ig

    IF Staining of Jurkat using 66224-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Positive IF/ICC detected inJurkat cells, human tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66224-1-Ig targets CD43 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen CD43 fusion protein Ag23595 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sialophorin
Synonyms Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein, CD43 cytoplasmic tail
Calculated Molecular Weight 400 aa, 43 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC012350
Gene Symbol CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_2881615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16150
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

实验方案

Product Specific Protocols
WB protocol for CD43 antibody 66224-1-IgDownload protocol
IHC protocol for CD43 antibody 66224-1-IgDownload protocol
IF protocol for CD43 antibody 66224-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols