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CD43 Monoclonal antibody, PBS Only

CD43 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A11D6

Cat no : 66224-1-PBS

Print datasheet

Synonyms

Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein, CD43 cytoplasmic tail

验证数据展示

  • WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    WB analysis of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66224-1-Ig (CD43 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IHC staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66224-1-Ig (CD43 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD43 antibody included
  • IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    IF Staining of human tonsillitis using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 66224-1-Ig (CD43 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    IF Staining of Jurkat using 66224-1-Ig (same clone as 66224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 66224-1-Ig (CD43 antibody) at dilution of 1:400 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66224-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

产品信息

66224-1-PBS targets CD43 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD43 fusion protein Ag23595 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name sialophorin
Synonyms Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein, CD43 cytoplasmic tail
Calculated Molecular Weight 400 aa, 43 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC012350
Gene Symbol CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_2881615
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16150
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.