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验证数据展示

  • IF Staining of human tonsillitis using CL488-66224

    IF Staining of human tonsillitis using CL488-66224

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66224 (CD43 antibody) at dilution of 1:50.

  • IF Staining of human tonsillitis using CL488-66224

    IF Staining of human tonsillitis using CL488-66224

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-66224 (CD43 antibody) at dilution of 1:50.

CoraLite® Plus 488-conjugated CD43 Monoclonal antibody

CD43 Monoclonal Antibody for IF-P
Cat No. CL488-66224

产品说明书

CloneNo. 2A11D6

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF-P

别名

CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66224-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66224 targets CD43 in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 CD43 fusion protein Ag23595 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 sialophorin
别名 CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN
计算分子量 400 aa, 43 kDa
观测分子量 115 kDa
GenBank蛋白编号BC012350
基因名称 CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_2883288
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16150
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD43 antibody CL488-66224Download protocol
Standard Protocols
Click here to view our Standard Protocols