验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
Positive FC detected in | human PBMCs |
推荐稀释比
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
ELISA | See 1 publications below |
产品信息
65150-1-Ig targets CD45RO in IHC, IF-P, FC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IF-P, FC Application Description |
Cited Applications | ELISA |
Tested Reactivity | human |
Immunogen | IL-2 dependent T cell line, CA1 种属同源性预测 |
Host / Isotype | Mouse / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | protein tyrosine phosphatase, receptor type, C |
Synonyms | CD45, B220, LCA, L CA, GP180 |
GenBank Accession Number | BC014239 |
Gene Symbol | CD45 |
Gene ID (NCBI) | 5788 |
ENSEMBL Gene ID | ENSG00000081237 |
RRID | AB_2881476 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08575 |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).
实验方案
Product Specific Protocols | |
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IHC protocol for CD45RO antibody 65150-1-Ig | Download protocol |
IF protocol for CD45RO antibody 65150-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |