验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, A549 cells, HEK-293 cells, human cerebellum tissue, pig kidney tissue, mouse kidney tissue, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy CNOT1 Ready-To-Use IHC Kit. CNOT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66507-1-Ig targets CNOT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Immunogen | CNOT1 fusion protein Ag5623 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CCR4-NOT transcription complex, subunit 1 |
| Synonyms | AD 005, CCR4 associated factor 1, CDC39, CNOT1, hNOT1, KIAA1007, NOT1, NOT1H |
| Calculated Molecular Weight | 267 kDa |
| Observed Molecular Weight | 240-250 kDa |
| GenBank Accession Number | BC040523 |
| Gene Symbol | CNOT1 |
| Gene ID (NCBI) | 23019 |
| RRID | AB_2881870 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | A5YKK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT1 antibody 66507-1-Ig | Download protocol |
| IHC protocol for CNOT1 antibody 66507-1-Ig | Download protocol |
| IF protocol for CNOT1 antibody 66507-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.
| ||
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Cell Rep The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation |
