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CoraLite® Plus 488-conjugated Cardiac Troponin I Monoclonal antibody

Cardiac Troponin I Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D5D6

Cat no : CL488-66376

Print datasheet

Synonyms

Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle

验证数据展示

  • IF Staining of mouse heart using CL488-66376

    IF Staining of mouse heart using CL488-66376

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Cardiac Troponin I antibody (CL488-66376, Clone: 1D5D6 ) at dilution of 1:200, CoraLite® Plus 647 smooth muscle actin specific antibody (CL647-67735, Clone: 1E9A11, magenta), CoraLite®594 N-cadherin antibody (CL594-22018, red). DAPI (blue).

经过测试的应用

Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy TNNI3 Ready-To-Use IHC Kit. TNNI3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66376-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66376 targets Cardiac Troponin I in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Cardiac Troponin I fusion protein Ag16758 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name troponin I type 3 (cardiac)
Synonyms Cardiac troponin I, CMD2A, CMH7, cTnI, RCM1, TNNC1, TNNI3, troponin I type 3 (cardiac), Troponin I, cardiac muscle
Calculated Molecular Weight 210 aa, 24 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC096165
Gene Symbol Cardiac Troponin I
Gene ID (NCBI) 7137
RRIDAB_2883322
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19429
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Troponin I (TnI) is the inhibitory subunit of troponin, the structural protein involved in the regulation of striated muscle contraction. Cardiac Troponin I (cTnI) is produced by cardiac muscle. Two other TnI isoforms (slow sTnI and fast sTnI) are produced by slow-twitch and fast-twitch skeletal muscles, respectively. cTnI has been considered as one of the most specific and sensitive markers of myocardial damage like acute myocardial infarction (AMI) for decades. Detection of cTnI in serum is widely accepted as a diagnostic tool in acute coronary syndromes. This antibody is specific to cTnI, but not cross-react with skeletal TnI.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cardiac Troponin I antibody CL488-66376Download protocol
Standard Protocols
Click here to view our Standard Protocols