验证数据展示
经过测试的应用
| Positive WB detected in | Human cerebellum tissue, human brain tissue, pig liver tissue, rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66091-1-Ig targets FKBP2 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, rat, pig samples.
| Tested Applications | WB, IHC, ELISA, FC (Intra) Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat, pig |
| Cited Reactivity | human |
| Immunogen | FKBP2 fusion protein Ag19113 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | FK506 binding protein 2, 13kDa |
| Synonyms | FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8 |
| Calculated Molecular Weight | 142 aa, 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC003384 |
| Gene Symbol | FKBP2 |
| Gene ID (NCBI) | 2286 |
| RRID | AB_11232416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P26885 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBP2 antibody 66091-1-Ig | Download protocol |
| IHC protocol for FKBP2 antibody 66091-1-Ig | Download protocol |
| FC protocol for FKBP2 antibody 66091-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
