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验证数据展示

  • WB analysis of human cerebellum using 66091-1-Ig (same clone as 66091-1-PBS)

    WB analysis of human cerebellum using 66091-1-Ig (same clone as 66091-1-PBS)

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66091-1-Ig (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

    IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

    IHC staining of human prostate cancer using 66091-1-Ig (same clone as 66091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66091-1-Ig (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66091-1-Ig (same clone as 66091-1-PBS)

    FC experiment of HepG2 using 66091-1-Ig (same clone as 66091-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug FKBP2 Monoclonal antibody (66091-1-Ig, Clone:2H7E8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66091-1-PBS in a different storage buffer formulation.

FKBP2 Monoclonal antibody, PBS Only

FKBP2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66091-1-PBS

产品说明书

CloneNo. 2H7E8

宿主/亚型

Mouse / IgG1

种属反应性

human, rat, pig

应用

WB, IHC, Indirect ELISA

别名

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66091-1-PBS targets FKBP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原 FKBP2 fusion protein Ag19113 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 FK506 binding protein 2, 13kDa
别名 FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8
计算分子量 142 aa, 16 kDa
观测分子量 16 kDa
GenBank蛋白编号BC003384
基因名称 FKBP2
Gene ID (NCBI) 2286
RRIDAB_11232416
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP26885
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).