验证数据展示
经过测试的应用
| Positive WB detected in | Human cerebellum tissue, human brain tissue, pig liver tissue, rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66091-1-Ig targets FKBP2 in WB, IHC, ELISA, FC (Intra) applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IHC, ELISA, FC (Intra) Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | FKBP2 fusion protein Ag19113 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | FK506 binding protein 2, 13kDa |
| 别名 | FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 2H7E8 |
| 计算分子量 | 142 aa, 16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC003384 |
| 基因名称 | FKBP2 |
| Gene ID (NCBI) | 2286 |
| RRID | AB_11232416 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P26885 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for FKBP2 antibody 66091-1-Ig | Download protocol |
| IHC protocol for FKBP2 antibody 66091-1-Ig | Download protocol |
| FC protocol for FKBP2 antibody 66091-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
