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验证数据展示

  • IF Staining of HeLa using CL594-67133

    IF Staining of HeLa using CL594-67133

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated FLNA antibody (CL594-67133, Clone: 1G4H3 ) at dilution of 1:400.

CoraLite®594-conjugated FLNA Monoclonal antibody

FLNA Monoclonal Antibody for IF/ICC
Cat No. CL594-67133
CloneNo. 1G4H3

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

Synonyms

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy FLNA Ready-To-Use IHC Kit. FLNA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67133-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67133 targets FLNA in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen FLNA fusion protein Ag23291 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name filamin A, alpha (actin binding protein 280)
Synonyms ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2
Calculated Molecular Weight 2647 aa, 280 kDa
Observed Molecular Weight 280 kDa
GenBank Accession NumberBC014654
Gene Symbol FLNA
Gene ID (NCBI) 2316
RRIDAB_2883639
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21333
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID: 29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID: 29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH).(PMID: 29062687)

实验方案

Product Specific Protocols
IF protocol for CL594 FLNA antibody CL594-67133Download protocol
Standard Protocols
Click here to view our Standard Protocols