CoraLite® Plus 488-conjugated FLNA Monoclonal antibody
FLNA Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G4H3
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy FLNA Ready-To-Use IHC Kit. FLNA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67133-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67133 targets FLNA in IF/ICC applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | FLNA fusion protein Ag23291 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | filamin A, alpha (actin binding protein 280) |
Synonyms | ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2 |
Calculated Molecular Weight | 2647 aa, 280 kDa |
Observed Molecular Weight | 280 kDa |
GenBank Accession Number | BC014654 |
Gene Symbol | FLNA |
Gene ID (NCBI) | 2316 |
RRID | AB_2883398 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P21333 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID: 29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID: 29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 FLNA antibody CL488-67133 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |