Glucocorticoid receptor Monoclonal antibody

Glucocorticoid receptor Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1D9A9

Cat no : 66904-1-Ig

Print datasheet

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1



经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, Jurkat cells, K-562 cells, PC-12 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66904-1-Ig targets Glucocorticoid receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Immunogen Glucocorticoid receptor fusion protein Ag28431 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Synonyms GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC015610
Gene Symbol Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_2857354
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04150
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

实验方案

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IHC protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IF protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Antioxidants (Basel)

Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress

Authors - Wenji Ren
mouseWB

Am J Physiol Cell Physiol

Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture.

Authors - Lisa M Cooper
mouseIF

Antioxidants (Basel)

Dim Blue Light at Night Induces Spatial Memory Impairment in Mice by Hippocampal Neuroinflammation and Oxidative Stress

Authors - Qi Liu