Glucocorticoid receptor Monoclonal antibody
Glucocorticoid receptor Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1D9A9
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, Jurkat cells, K-562 cells, PC-12 cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66904-1-Ig targets Glucocorticoid receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | mouse |
Immunogen | Glucocorticoid receptor fusion protein Ag28431 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
Synonyms | GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1 |
Calculated Molecular Weight | 86 kDa |
Observed Molecular Weight | 97 kDa |
GenBank Accession Number | BC015610 |
Gene Symbol | Glucocorticoid receptor |
Gene ID (NCBI) | 2908 |
RRID | AB_2857354 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P04150 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Glucocorticoid receptor antibody 66904-1-Ig | Download protocol |
IHC protocol for Glucocorticoid receptor antibody 66904-1-Ig | Download protocol |
IF protocol for Glucocorticoid receptor antibody 66904-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Antioxidants (Basel) Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress | ||
Am J Physiol Cell Physiol Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture. | ||
Antioxidants (Basel) Dim Blue Light at Night Induces Spatial Memory Impairment in Mice by Hippocampal Neuroinflammation and Oxidative Stress |