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CoraLite® Plus 488-conjugated Glucocorticoid receptor Monoclonal antibody

Glucocorticoid receptor Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D9A9

Cat no : CL488-66904

Print datasheet

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

验证数据展示

  • IF Staining of mouse brain using CL488-66904

    IF Staining of mouse brain using CL488-66904

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Glucocorticoid receptor antibody (CL488-66904, Clone: 1D9A9 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL488-66904

    IF Staining of mouse brain using CL488-66904

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 Glucocorticoid receptor antibody (CL488-66904, Clone: 1D9A9 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-66904

    FC experiment of HeLa using CL488-66904

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Glucocorticoid receptor (CL488-66904, Clone:1D9A9) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66904-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66904 targets Glucocorticoid receptor in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity Human, mouse, rat
Immunogen Glucocorticoid receptor fusion protein Ag28431 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Synonyms GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC015610
Gene Symbol Glucocorticoid receptor
Gene ID (NCBI) 2908
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04150
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-66904Download protocol
FC protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-66904Download protocol
Standard Protocols
Click here to view our Standard Protocols