Glucocorticoid receptor Monoclonal antibody

Glucocorticoid receptor Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66904-1-Ig

产品说明书

CloneNo. 1D9A9

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, MCF-7 cells, Jurkat cells, K-562 cells, PC-12 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66904-1-Ig targets Glucocorticoid receptor in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 Human, mouse, rat
文献引用反应性mouse
免疫原 Glucocorticoid receptor fusion protein Ag28431 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
别名 GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1
计算分子量 86 kDa
观测分子量 97 kDa
GenBank蛋白编号BC015610
基因名称 Glucocorticoid receptor
Gene ID (NCBI) 2908
RRIDAB_2857354
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04150
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

实验方案

Product Specific Protocols
WB protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IHC protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
IF protocol for Glucocorticoid receptor antibody 66904-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Antioxidants (Basel)

Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress

Authors - Wenji Ren
mouseWB

Am J Physiol Cell Physiol

Dual-Specificity Phosphatase 29 is Induced During Neurogenic Skeletal Muscle Atrophy and Attenuates Glucocorticoid Receptor Activity in Muscle Cell Culture.

Authors - Lisa M Cooper
mouseWB

Heliyon

Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models

Authors - Fei Fei
mouseIF

Antioxidants (Basel)

Dim Blue Light at Night Induces Spatial Memory Impairment in Mice by Hippocampal Neuroinflammation and Oxidative Stress

Authors - Qi Liu
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