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Glucocorticoid receptor Monoclonal antibody, PBS Only

Glucocorticoid receptor Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D9A9

Cat No : 66904-1-PBS

Print datasheet

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

验证数据展示

  • WB analysis using 66904-1-Ig (same clone as 66904-1-PBS)

    WB analysis using 66904-1-Ig (same clone as 66904-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    IHC staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66904-1-Ig (Glucocorticoid receptor antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NR3C1 antibody included
  • IF Staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    IF Staining of mouse brain using 66904-1-Ig (same clone as 66904-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Glucocorticoid receptor antibody (66904-1-Ig, Clone: 1D9A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66904-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy NR3C1 Ready-To-Use IHC Kit. NR3C1 primary antibody included.

产品信息

66904-1-PBS targets Glucocorticoid receptor in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Glucocorticoid receptor fusion protein Ag28431 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Synonyms GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC015610
Gene Symbol NR3C1
Gene ID (NCBI) 2908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04150
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).