验证数据展示
经过测试的应用
Positive IF-P detected in | mouse brain tissue |
Positive FC (Intra) detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66904 targets Glucocorticoid receptor in IF-P, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
经测试应用 | IF-P, FC (Intra) Application Description |
经测试反应性 | Human, mouse, rat |
免疫原 | Glucocorticoid receptor fusion protein Ag28431 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) |
别名 | GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1 |
计算分子量 | 86 kDa |
观测分子量 | 97 kDa |
GenBank蛋白编号 | BC015610 |
基因名称 | Glucocorticoid receptor |
Gene ID (NCBI) | 2908 |
RRID | AB_2934749 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P04150 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].
实验方案
Product Specific Protocols | |
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IF protocol for CL594 Glucocorticoid receptor antibody CL594-66904 | Download protocol |
FC protocol for CL594 Glucocorticoid receptor antibody CL594-66904 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |