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验证数据展示

  • WB analysis using 67009-1-Ig

    WB analysis using 67009-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-12 using 67009-1-Ig

    IF Staining of PC-12 using 67009-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (67009-1-Ig, Clone: 1A7E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

KIF5A Monoclonal antibody

KIF5A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67009-1-Ig
CloneNo. 1A7E3

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue
Positive IF/ICC detected inPC-12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67009-1-Ig targets KIF5A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen KIF5A fusion protein Ag15682 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name kinesin family member 5A
Synonyms D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10
Calculated Molecular Weight 1032 aa, 117 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC146670
Gene Symbol KIF5A
Gene ID (NCBI) 3798
RRIDAB_2882326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12840
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

实验方案

Product Specific Protocols
WB protocol for KIF5A antibody 67009-1-IgDownload protocol
IF protocol for KIF5A antibody 67009-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols