CoraLite® Plus 488-conjugated KIF5A Monoclonal antibody
KIF5A Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A7E3
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | PC-12 cells |
For other applications, we recommend the unconjugated version of this antibody, 67009-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67009 targets KIF5A in IF/ICC applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat, pig |
| Immunogen | KIF5A fusion protein Ag15682 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | kinesin family member 5A |
| Synonyms | D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10 |
| Calculated Molecular Weight | 1032 aa, 117 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC146670 |
| Gene Symbol | KIF5A |
| Gene ID (NCBI) | 3798 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q12840 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 KIF5A antibody CL488-67009 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |