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验证数据展示

  • WB analysis using 67009-1-Ig

    WB analysis using 67009-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67009-1-Ig (KIF5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-12 using 67009-1-Ig

    IF Staining of PC-12 using 67009-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using KIF5A antibody (67009-1-Ig, Clone: 1A7E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

KIF5A Monoclonal antibody

KIF5A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67009-1-Ig

产品说明书

CloneNo. 1A7E3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue, pig brain tissue, rat cerebellum tissue, mouse brain tissue
Positive IF/ICC detected inPC-12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67009-1-Ig targets KIF5A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 KIF5A fusion protein Ag15682 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 kinesin family member 5A
别名 D12S1889, KIF5A, kinesin family member 5A, Kinesin heavy chain isoform 5A, MY050, Neuronal kinesin heavy chain, NKHC, NKHC1, SPG10
计算分子量 1032 aa, 117 kDa
观测分子量 130 kDa
GenBank蛋白编号BC146670
基因名称 KIF5A
Gene ID (NCBI) 3798
RRIDAB_2882326
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ12840
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin superfamily proteins (KIFs) are microtubule-based molecular motors essential for the intracellular transport of various cargos, including organelles, proteins, and RNAs. KIF5A is expressed exclusively in neurons and transports neuronal cargoes into axons and dendrites. KIF5A mutations have been associated with Charcot-Marie-Tooth Type 2, an axonal peripheral neuropathy characterized by progressive loss of peripheral sensation and muscle wasting.

实验方案

Product Specific Protocols
WB protocol for KIF5A antibody 67009-1-IgDownload protocol
IF protocol for KIF5A antibody 67009-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols