验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells, T-47D cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66776 targets MFN1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | MFN1 fusion protein Ag4890 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | mitofusin 1 |
Synonyms | mitofusin 1, hfzo2, hfzo1, Fzo homolog, EC:3.6.5.- |
Calculated Molecular Weight | 741 aa, 84 kDa |
Observed Molecular Weight | 84 kDa |
GenBank Accession Number | BC040557 |
Gene Symbol | MFN1 |
Gene ID (NCBI) | 55669 |
RRID | AB_2883626 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q8IWA4 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
实验方案
Product Specific Protocols | |
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IF protocol for CL594 MFN1 antibody CL594-66776 | Download protocol |
FC protocol for CL594 MFN1 antibody CL594-66776 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |