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PDH E1 Alpha Monoclonal antibody

PDH E1 Alpha Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2B3C10

Cat no : 66119-1-Ig

Print datasheet

Synonyms

PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1



经过测试的应用

Positive WB detected inHEK-293 cells, HEK293 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDHA1 Ready-To-Use IHC Kit. PDHA1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66119-1-Ig targets PDH E1 Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human
Cited Reactivityhuman
Immunogen PDH E1 Alpha fusion protein Ag12556 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name pyruvate dehydrogenase (lipoamide) alpha 1
Synonyms PDH E1α, PDHA1, PDHA 1, PDHA, EC:1.2.4.1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC002406
Gene Symbol PDHA1
Gene ID (NCBI) 5160
RRIDAB_2881518
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08559
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

实验方案

Product Specific Protocols
WB protocol for PDH E1 Alpha antibody 66119-1-IgDownload protocol
IHC protocol for PDH E1 Alpha antibody 66119-1-IgDownload protocol
IF protocol for PDH E1 Alpha antibody 66119-1-IgDownload protocol
IP protocol for PDH E1 Alpha antibody 66119-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Authors - Xiaoyu Ma

J Cell Biol

PINK1 disables the anti-fission machinery to segregate damaged mitochondria for mitophagy.

Authors - Kenneth R Pryde
humanWB

Cell Rep

A LON-ClpP Proteolytic Axis Degrades Complex I to Extinguish ROS Production in Depolarized Mitochondria.

Authors - Kenneth Robert Pryde
humanWB

Toxicol Appl Pharmacol

Sodium cantharidate induces Apoptosis in breast cancer cells by regulating energy metabolism via the protein phosphatase 5-p53 axis.

Authors - Jin-Long Pang
humanWB

Immunobiology

The mTOR-glycolytic pathway promotes T-cell immunobiology in oral lichen planus.

Authors - Fang Wang
humanWB,IF

Nat Metab

MAPK signalling-induced phosphorylation and subcellular translocation of PDHE1α promotes tumour immune evasion.

Authors - Yajuan Zhang
  • KD Validated