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RAB11A Monoclonal antibody

RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4A4C9

Cat no : 67902-1-Ig

Print datasheet

Synonyms

RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2

验证数据展示

  • WB analysis using 67902-1-Ig

    WB analysis using 67902-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 67902-1-Ig

    WB analysis using 67902-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of mouse brain using 67902-1-Ig

    WB analysis of mouse brain using 67902-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67902-1-Ig

    FC experiment of HeLa using 67902-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RAB11A Monoclonal antibody (67902-1-Ig, Clone:4A4C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A549 using 67902-1-Ig

    IF Staining of A549 using 67902-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inmouse brain tissue, A431 cells, A549 cells, HeLa cells, pig brain tissue, rat brain tissue, rat cerebellum tissue, HepG2 cells, K-562 cells, rabbit brain tissue
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RAB11A/B Ready-To-Use IHC Kit. RAB11A/B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67902-1-Ig targets RAB11A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Immunogen RAB11A fusion protein Ag8879 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RAB11A, member RAS oncogene family
Synonyms RAB11, Rab 11, RAB11A/B, Rab-11, EC:3.6.5.2
Calculated Molecular Weight 216 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC013348
Gene Symbol RAB11A
Gene ID (NCBI) 8766
RRIDAB_2918658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62491
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

实验方案

Product Specific Protocols
WB protocol for RAB11A antibody 67902-1-IgDownload protocol
IF protocol for RAB11A antibody 67902-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

A MYC-STAMBPL1-TOE1 positive feedback loop mediates EGFR stability in hepatocellular carcinoma

Authors - Hongli Zhang
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WB,IF

Cell Commun Signal

Atg5-deficient mesenchymal stem cells protect against non-alcoholic fatty liver by accelerating hepatocyte growth factor secretion

Authors - Caifeng Zhang
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