验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, fetal human brain tissue, pig brain tissue |
| Positive IHC detected in | human liver cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66339-1-Ig targets RAB5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, monkey |
| Immunogen | RAB5A fusion protein Ag16781 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | RAB5A, member RAS oncogene family |
| Synonyms | RAB5, RAB5A, Ras related protein Rab 5A |
| Calculated Molecular Weight | 215 aa, 24 kDa |
| Observed Molecular Weight | 24 kDa |
| GenBank Accession Number | BC001267 |
| Gene Symbol | RAB5A |
| Gene ID (NCBI) | 5868 |
| RRID | AB_2881719 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20339 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB5A antibody 66339-1-Ig | Download protocol |
| IHC protocol for RAB5A antibody 66339-1-Ig | Download protocol |
| IF protocol for RAB5A antibody 66339-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Matrix stiffness exacerbates the proinflammatory responses of vascular smooth muscle cell through the DDR1-DNMT1 mechanotransduction axis. | ||
Clin Ther Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019. | ||
J Exp Clin Cancer Res TFEB-driven autophagy potentiates TGF-β induced migration in pancreatic cancer cells. | ||
Commun Biol Arginine GlcNAcylation of Rab small GTPases by the pathogen Salmonella Typhimurium. | ||
PLoS Pathog ALIX and TSG101 are essential for cellular entry and replication of two porcine alphacoronaviruses | ||
Cell Rep Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina |
